Abstract Continuous flow electrophoresis (CFE) is a mild and high-resolving method for the separation of sensitive biomolecules like peptides and proteins. The resolution is decreased by several effects, of which density gradients are important since they lead to buoyancy-driven convection. A tool for the measurement of physical quantities that cause the separation process, a potentialgradient/conductivity-scanner has been developed. With this PC-scanner consecutive potential- and concentration profiles can be measured inside the CFE-chamber. Local current densities and local joule heat yielding density gradients are calculated from the measurement data. With the PC-scanner the influence of gravity on separations by CFE can be evaluated more easily by the knowledge of the thermal and electrical conditions inside the separation slit.


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    Title :

    A potentialgradient-conductivity-scanner for the investigation of effects leading to buoyancy-driven convection on continuous-flow-electrophoresis


    Contributors:
    Heinrich, J. (author) / Wagner, H. (author)

    Published in:

    Advances in Space Research ; 12 , 1 ; 385-392


    Publication date :

    1991-01-01


    Size :

    8 pages




    Type of media :

    Article (Journal)


    Type of material :

    Electronic Resource


    Language :

    English