Abstract The use of stereo microscopes is on the rise in many biotechnology fields, but there are certain issues that are unique to binocular stereo microscopes, such as required calibrations, because a microscope’s depth of focus is so small. In this paper, a new method that combines 2-D calibration and depth information is proposed to calibrate the binocular stereo microscope. First, based on the parameter estimate of a CCD or CMOS sensor, a calibration board is employed to acquire intrinsic and extrinsic parameters of the camera. Then, with images collected at different Z coordinates, the intrinsic and extrinsic parameters calibrated earlier are used to obtain the spatial coordinates of the calibration board by 2D reconstruction. Finally, a computed Z coordinate is compared with the actual Z coordinate in order to acquire the linear correction coefficient between the two and, thus, finish the final calibration. The experimental results show that the proposed method is both simple and practical, and the WCS (World Coordinate System) position recovered from the acquired calibration coefficient has an error of 0.0034mm along the X and Y axes and an error within 4.5% along the Z axis.


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    Title :

    Calibration Method of Stereo Microscope Based on Depth Correction


    Contributors:
    Wang, Yigang (author) / Jiang, Gangyi (author) / Yu, Mei (author) / Fan, Shengli (author)


    Publication date :

    2014-01-01


    Size :

    12 pages





    Type of media :

    Article/Chapter (Book)


    Type of material :

    Electronic Resource


    Language :

    English




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