Aim of this work was to evaluate stirred, single-use bioreactors by means of their heat transfer capabilities. Although this bioreactor concept is increasingly adapted by the biopharmaceutical industry, this technical aspect is currently underrepresented in the literature. In contrast to conventional, stainless-steel bioreactors, single-use systems provide some inherent properties that possibly affect heat transfer, such as the polymer film layer of the cultivation chamber and a limited heat transfer area. Therefore, commercially available reaction systems should be investigated. To assess the relevant systems, an adequate experimental procedure was developed. Generally, two types of methods are available: transient experiments, i.e., heating and cooling curves, can directly be applied to jacketed, stirred bioreactors with minimal modification. However, results derived from this approach are more difficult to evaluate, because of the dynamic system behavior. Secondly, steady state methods can be applied, which require an additional heat source. Often electrical heaters are used. In this work, a chemical alternative was established and applied up to a scale of 200 L: the exothermic decay of hydrogen peroxide. Another published work focused on a comprehensive study of thermal properties of stirred single-use bioreactors, where especially transient and steady state experiments were carried out in parallel and set into relation. Finally, a full-system heat transfer model of a stirred 500 L bioreactor was set up, considering all relevant peripheral components required for heat transfer. Single-use bioreactors are mainly used for mammalian cell cultures. The challenging requirements towards the reaction equipment imposed by microbial cultures can hardly be fulfilled by current systems. Nevertheless, these applications are industrially relevant and could generally benefit from the technology. One of the main challenges lies in the metabolic heat produced by the microbial cells. To investigate the biological side, a similar experimental approach was applied to E. coli fed-batch processes, but in a 5 L glass bioreactor. The present work, therefore, represents a contribution to both, the engineering characterization of single-use bioreactors and the related application of calorimetric methods to microbial processes.
Heat transfer in single-use bioreactors and E. coli bioprocesses
Wärmeübertragung in Single-Use Bioreaktoren und E. coli Bioprozessen
2021
Sonstige
Elektronische Ressource
Englisch
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